iPSで膵臓を器官形成：最新のセル東大医科研中内グループ

東大の中内さんのところからiPSを使ってラット膵臓をマウス体内に器官形成させたという極めて興奮すべき論文が出た。最新のセルである。

Generation of Rat Pancreas in Mouse by Interspecific Blastocyst Injection of Pluripotent Stem Cells

Cell, Volume 142, Issue 5, 787-799, 3 September 2010

Toshihiro Kobayashi, Tomoyuki Yamaguchi, Sanae Hamanaka, Megumi Kato-Itoh, Yuji Yamazaki, Makoto Ibata, Hideyuki Sato, Youn-Su Lee, Jo-ichi Usui, A.S. Knisely, Masumi Hirabayashi, Hiromitsu NakauchiSee Affiliations

Summary

The complexity of organogenesis hinders in vitro generation of organs derived from a patient's pluripotent stem cells (PSCs), an ultimate goal of regenerative medicine. Mouse wild-type PSCs injected into Pdx1(-/-) (pancreatogenesis-disabled) mouse blastocysts developmentally compensated vacancy of the pancreatic 'developmental niche', generating almost entirely PSC-derived pancreas. To examine the potential for xenogenic approaches in blastocyst complementation, we injected mouse or rat PSCs into rat or mouse blastocysts, respectively, generating interspecific chimeras and thus confirming that PSCs can contribute to xenogenic development between mouse and rat. The development of these mouse/rat chimeras was primarily influenced by host blastocyst and/or foster mother, evident by body size and species-specific organogenesis. We further injected rat wild-type PSCs into Pdx1(-/-)mouse blastocysts, generating normally functioning rat pancreas in Pdx1(-/-)mice. These data constitute proof of principle for interspecific blastocyst complementation and for generation in vivo of organs derived from donor PSCs using a xenogenic environment.